Anti-GM130抗体(ab32337)
Key features and details
- Rabbit polyclonal to GM130
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GM130抗体
参阅全部 GM130 一抗 -
描述
兔多克隆抗体to GM130 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to GM130 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32336) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). |
靶标
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功能
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
序列相似性
Belongs to the GOLGA2 family. -
结构域
Extended rod-like protein with coiled-coil domains. -
细胞定位
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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别名
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
图片
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Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab32337 被引用在 7 文献中.
- Bonsergent E et al. Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells. Nat Commun 12:1864 (2021). PubMed: 33767144
- Shi L et al. Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34155143
- Ding C et al. Exosomal Circ-MEMO1 Promotes the Progression and Aerobic Glycolysis of Non-small Cell Lung Cancer Through Targeting MiR-101-3p/KRAS Axis. Front Genet 11:962 (2020). PubMed: 33005174
- Lei L & Mou Q Exosomal taurine up-regulated 1 promotes angiogenesis and endothelial cell proliferation in cervical cancer. Cancer Biol Ther 21:717-725 (2020). PubMed: 32432954
- Wang J et al. Exosome-Mediated Transfer of lncRNA HOTTIP Promotes Cisplatin Resistance in Gastric Cancer Cells by Regulating HMGA1/miR-218 Axis. Onco Targets Ther 12:11325-11338 (2019). PubMed: 31908497
- Maier PJ et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014). ICC/IF . PubMed: 24668805
- O'Sullivan NC et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Drosophila melanogaster . PubMed: 22543973