Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (ThermoScientific 4-20% precise tris-glycine)
Sample
Rat Tissue lysate - nuclear (Nucleus of the solitary tract and ileum)
Specification
Nucleus of the solitary tract and ileum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immunoresearch peroxidase-conjugated donke
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
40 minute(s) and 0 second(s)
Positive control
Beta actin
Additional data
Additional Notes
I loaded a range of total protein amounts 10, 20, 30, and 90 ug.
Abcam response
The review is of a previously untested species, rat. The customer participated in our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 18 2014