Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
HEK 293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
Cells lysed in ChIP lysis buffer, crosslinked with 1% formaldehyde
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 23 2018