Application
Other - Do not use
Sample
Arabidopsis thaliana Tissue sections (True leaf from 8-day old seedling)
Secondary antibody
Name
Non-Abcam antibody was used: 18 nm Gold-AffiniPure Donkey Anti-Goat IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Gold 18nm
Host species: Donkey
Clonality: Polyclonal
Conjugation: Gold 18nm
Dilution
1/15
Additional data
Notes
Arabidopsis thaliana transgenic plant expressing GFP fused to an Endoplasmic Reticulum (ER) marker. The ab5450 antibody labels ER-derived aggregates specifically. No label is observed in chloroplasts (C), mitochondria (M) nor in the Golgi (G). The sample was prepared by cryofixation and embedding in Lowicryl HM20 resin. The image was taken in a JEOL 1010 transmission electron microscope at 20,000¿ magnification.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Narciso Campos
Verified customer
提交于 Jun 25 2013