Application
Western blot
Sample
Human Recombinant protein (Recombinant GFP-Ku70 protein)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
0.1 µg
Specification
Recombinant GFP-Ku70 protein
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey Anti-chicken IgY antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 680LT
Detection
Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 95 kDa
Positive control
Recombinant GFP-Ku70 protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 11 2017