Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 mouse embryo)
Permeabilization
Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Specification
E10.5 mouse embryo
Blocking step
NOVOCASTRA protein block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: donkey anti-mouse 488 (Invitrogen)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Embryos were electroporated with Plasmid 13776: pCAG-Cre:GFP from Addgene and then fixed with 4% PFA overnight at 4 degree. Samples were embedded in gelatin and sectioned using a cryostat.
GFP can be detected using anti-GFP ab13970, but not anti-GFP ab1218.
Abcam response
Thank you for sharing this data with us. ab1218 has previously been used to detect GFP in IHC-Fr by other customers, please see the abreviews and references on our datasheet. However, your data shows that this antibody is not suitable for IHC-Fr, under these conditions. Abcam would welcome feedback from other customers currently using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 25 2014