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We have not tested ab171581 with a GFP fusion protein (purified or in cell extract) before. The epitopes for the capture and detector antibodies are also not known because both are made using full length immunogens. However, both antibodies alone have detected GFP fusion proteins in other applications (WB, IP), so it is encouraging that at least the individual antibodies alone are capable of detecting GFP fusion proteins.
Kevin Hanson Abcam Scientific Support
回复于 Feb 23 2016