Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
No good for this application, in my hands.
Three areas are seen in the composite image ( upper is this Ab, lower is a "gold standard" anti GFAP Ab): white matter, grey matter and exit zone of progenitor olfactory neurones.
None of these areas are positive for ab93251.
Region shown is the lateral ventricle showing the exit zone for progenitor olfactory neurones.
Three areas are seen in the composite image ( upper is this Ab, lower is a "gold standard" anti GFAP Ab): white matter, grey matter and exit zone of progenitor olfactory neurones.
None of these areas are positive for ab93251.
Region shown is the lateral ventricle showing the exit zone for progenitor olfactory neurones.
Abcam response
Thank you for submitting an Abreview. ab93251 apparently does not seem to work well in this experimental procedure and specie, under these conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 May 16 2012