Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification
total larvae, 4 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat IgG
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 48 kDa Non-specific: 40, 52 kDa
Exposure
15 second(s)
Positive control
gapdh
Additional data
Additional Notes
arrow indicates band of interest
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Evgenia Salta
Verified customer
提交于 Oct 27 2014