Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (neuronal indicution))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells (neuronal indicution)
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Blocking solution without Trtion
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Cells were induced in neuronal lineage and express GFAP protein. Control without primary antibody and on non differentiated cells were negative.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Antoine Veron
Verified customer
提交于 Sep 20 2016