Application
IHC - Wholemount
Sample
Mouse Tissue (Colon)
Specification
Colon
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 4% NGS, 1% BSA, and 0.4% Triton in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat anti-Chicken
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This antibody was used in free floating IHC of mouse colon longitudinal muscle myenteric plexus preparations. Tissue was post-fixed 16 hours with Zamboni's fixative at 4 degrees Celsius and washed before dissecting. Enteric glial cell processes are labeled very well with minimal background.
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提交于 Jun 13 2016