Application
Western blot
Sample
Mouse Cell lysate - nuclear (ES cells)
Loading amount
20 µg
Specification
ES cells
Treatment
1 µM retinoic acid for 1.5 days
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/80
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (Santa Cruz)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: around 50 kDa Non-specific: around 120 kDa
Additional data
Additional Notes
1. Cell extract of HAmGCNF expressing COS1 cells.
2. 20 ug nuclear extract of Dox+ control mES cells treated with RA for 1.5 days.
3. 20 ug nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days.
4. 40 ug nuclear extract of Dox+ control mES cells treated with RA for 1.5 days.
5. 40 ug nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days.
2. 20 ug nuclear extract of Dox+ control mES cells treated with RA for 1.5 days.
3. 20 ug nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days.
4. 40 ug nuclear extract of Dox+ control mES cells treated with RA for 1.5 days.
5. 40 ug nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days.
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提交于 Mar 25 2013