Application
Flow Cytometry
Sample
Human Cell (Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l)
Specification
Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l
Preparation
Cell harvesting/tissue preparation method: U937 cells were culture in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspend to 200 000cells in 100ul
Sample buffer: PBS + 0.1% FCS
Sample buffer: PBS + 0.1% FCS
Fixation
No fixation
Permeabilization
No
Gating Strategy
FS - SS
Other product details
Concentration
10 µg/ml
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% FCS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse PE (Jackson)
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
PubMed ID
21156793: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. David Sanchez Martin
Verified customer
提交于 Jan 24 2011