Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in 0.1% PBST
Dilution
1/25
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Abcam response
This antibody has so far not been tested with rat samples yet. A high homology of the rat protein with the immunogen of this antibody suggests that it could work reliably. We would like to encourage other researchers to submit an Abreview with their rat samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
提交于 Dec 21 2016