Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bronchial epithelial cells overexpressing GBA2)
Permeabilization
Yes - 0.1% PBS-Tween
Specification
Bronchial epithelial cells overexpressing GBA2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/10% normal goat serum/0.3M glycin
Concentration
5 µg/ml
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Alexa Fluor 647
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Massimo Aureli
Verified customer
提交于 Apr 25 2016