重组Anti-GAS2抗体[EPR1742(2)] (ab109762)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1742(2)] to GAS2
- Suitable for: WB, IHC-P
- Reacts with: Human
概述
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产品名称
Anti-GAS2抗体[EPR1742(2)] -
描述
兔单克隆抗体[EPR1742(2)] to GAS2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1742(2) -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109762于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process. -
组织特异性
Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen. -
序列相似性
Belongs to the GAS2 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 GAR domain. -
发展阶段
Specifically expressed at growth arrest. -
翻译后修饰
Cleaved, during apoptosis, on a specific aspartic residue by caspases.
Phosphorylated on serine residues during the G0-G1 transition phase. -
细胞定位
Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 2620 Human
- Entrez Gene: 14453 Mouse
- Omim: 602835 Human
- SwissProt: O43903 Human
- SwissProt: P11862 Mouse
- Unigene: 632151 Human
- Unigene: 207360 Mouse
- Unigene: 386823 Mouse
see all -
别名
- GAS 2 antibody
- GAS-2 antibody
- Gas2 antibody
see all
图片
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All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109762 被引用在 4 文献中.
- Ma W et al. Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC. Mol Oncol 16:3720-3734 (2022). PubMed: 36054080
- Sun N et al. DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients. Pediatr Investig 5:106-111 (2021). PubMed: 34179706
- Shukla S et al. Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance. Cancer Cell 32:792-806.e7 (2017). PubMed: 29153843
- Sun L et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). PubMed: 26358320