Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (Total cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
Total cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-rabbit IgG (H+L) 0.5mg
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LiCoR
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Positive control
C. elegans were fed with OP50 (regular diet) and comma (another diet for C. elegans) and were compared.
Additional data
Additional Notes
Li-COR. antibody was used in 2.5% skim milk- 1:1000.
C. elegans were fed with OP50 (regular diet) and comma (another diet for C. elegans) and were compared.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 25 2016