Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (Acrylamide gel, 10%)
Sample
Human Tissue lysate - whole (brain (frontal cortex))
Specification
brain (frontal cortex)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Tween 0,1%
Dilution
1/2000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Bands
Specific: 36 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 31 2013