The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 17 kDa).
We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.