Application
IHC - Wholemount
Sample
Rat Tissue (Inhibitory Neurons)
Specification
Inhibitory Neurons
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (PBS w/ 0.05% Triton)
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) *Highl
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Website error: IHC-FrFl was performed and used for IF.
Fixative: Paraformaldehyde
Permeablization: x1 for 10min in PBS w/ 0.5% Triton
Blocking: 10% Serum for 1 hour @ Room Temp
Center of image shows decent staining for GAD in axons
Abcam response
Thank you for taking part in our AbTrial testing program. Our Scientific Support team has been in contact with this reviewer and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 18 2015