Anti-GAD65 + GAD67抗体(ab49832)
Key features and details
- Rabbit polyclonal to GAD65 + GAD67
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GAD65 + GAD67抗体
参阅全部 GAD65 + GAD67 一抗 -
描述
兔多克隆抗体to GAD65 + GAD67 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide:
K-DIDFLIEEIERLGQDL
conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (2) |
1/1000 - 1/2000.
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说明 |
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IHC-P
1/1000 - 1/2000. |
靶标
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相关性
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
细胞定位
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
数据库链接
- Entrez Gene: 24379 Rat
- Entrez Gene: 24380 Rat
- SwissProt: P18088 Rat
- SwissProt: Q05683 Rat
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别名
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
图片
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.
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ab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab49832 被引用在 29 文献中.
- Giehrl-Schwab J et al. Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons. EMBO Mol Med 14:e14797 (2022). PubMed: 35373464
- Favrais G et al. Partial protective effects of melatonin on developing brain in a rat model of chorioamnionitis. Sci Rep 11:22167 (2021). PubMed: 34773065
- Grippo RM et al. Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding. Curr Biol 30:196-208.e8 (2020). PubMed: 31902720
- Chang W et al. PTH hypersecretion triggered by a GABAB1 and Ca2+-sensing receptor heterocomplex in hyperparathyroidism. Nat Metab 2:243-255 (2020). PubMed: 32694772
- Meyer G et al. Cajal-Retzius neurons are required for the development of the human hippocampal fissure. J Anat 235:569-589 (2019). PubMed: 30861578