Anti-GABA抗体[5A9] (ab86186)
Key features and details
- Mouse monoclonal [5A9] to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
概述
-
产品名称
Anti-GABA抗体[5A9]
参阅全部 GABA 一抗 -
描述
小鼠单克隆抗体[5A9] to GABA -
宿主
Mouse -
特异性
ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used. -
经测试应用
适用于: IHC-P, ICC/IF, IHC-FoFrmore details -
种属反应性
与反应: Species independent -
免疫原
GABA coupled to BSA with glutaraldehyde
-
常规说明
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein G purified -
Primary antibody说明
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
克隆
单克隆 -
克隆编号
5A9 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use at an assay dependent concentration.
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
靶标
-
相关性
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
细胞定位
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
别名
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
图片
-
ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).
Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.
-
Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab86186 被引用在 11 文献中.
- Scheuer T et al. Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons. Oxid Med Cell Longev 2022:8469756 (2022). PubMed: 35663195
- Jia X et al. Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats. J Neurochem 158:373-390 (2021). PubMed: 33950542
- Yu XJ et al. Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension. Front Neurosci 15:805070 (2021). PubMed: 34938159
- Trujillo CA et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
- Chéreau R et al. Dynamic perceptual feature selectivity in primary somatosensory cortex upon reversal learning. Nat Commun 11:3245 (2020). PubMed: 32591523