Anti-GABA A Receptor beta 3/GABRB3抗体[N87/25] (ab98968)
Key features and details
- Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3
- Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-GABA A Receptor beta 3/GABRB3抗体[N87/25]
参阅全部 GABA A Receptor beta 3/GABRB3 一抗 -
描述
小鼠单克隆抗体[N87/25] to GABA A Receptor beta 3/GABRB3 -
宿主
Mouse -
特异性
Does not cross react with GABA A Receptor Beta 2 or Beta 1. -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Orangutan -
免疫原
Fusion protein corresponding to Mouse GABA A Receptor beta 3/GABRB3 aa 350-450.
Database link: P63080 -
阳性对照
- WB: Rat brain lysate. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse brain tissue.
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常规说明
The clone number has been updated from S87-25 to N87/25, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N87/25 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/1000.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
说明 |
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IHC-Fr
1/1000. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 55 kDa. Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
靶标
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功能
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. -
疾病相关
Epilepsy, childhood absence 5 -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. -
细胞定位
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2562 Human
- Entrez Gene: 14402 Mouse
- Entrez Gene: 24922 Rat
- Omim: 137192 Human
- SwissProt: P28472 Human
- SwissProt: P63080 Mouse
- SwissProt: P63079 Rat
- Unigene: 302352 Human
see all -
别名
- ECA5 antibody
- GABA alpha receptor beta-2 subunit antibody
- GABA(A) receptor subunit beta-3 antibody
see all
图片
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Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 55 kDaBlock: 1.5% BSA for 30 minutes at RT.
Incubation with primary antibody for 2 hours at RT.
Incubation with secondary antibody for 1 hour at RT.
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ab98968 staining GABA A Receptor beta 3/GABRB3 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Tissue was fixed in 10% formalin for 12-24 hours at RT. Primary Antibody: ab98968 at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
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Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab98968 (red line).
The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 minutes at 22ºC.
The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab98968 被引用在 14 文献中.
- Menzikov SA et al. Physiological Role of ATPase for GABAA Receptor Resensitization. Int J Mol Sci 23:N/A (2022). PubMed: 35628132
- Chu MC et al. Generational synaptic functions of GABAA receptor β3 subunit deteriorations in an animal model of social deficit. J Biomed Sci 29:51 (2022). PubMed: 35821032
- Liu Q et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
- Farmer GE et al. AT1a-dependent GABAA inhibition in the MnPO following chronic intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol 321:R469-R481 (2021). PubMed: 34189959
- Victor AK et al. Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility. Front Mol Neurosci 14:747855 (2021). PubMed: 34776864