Anti-GABRA4抗体(ab117080)
Key features and details
- Goat polyclonal to GABRA4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GABRA4抗体
参阅全部 GABRA4 一抗 -
描述
山羊多克隆抗体to GABRA4 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human GABRA4 aa 300-400 (internal sequence) (Cysteine residue).
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阳性对照
- Human Frontal Cortex lysate and Human Brain, Cortex tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2557 Human
- Omim: 137141 Human
- SwissProt: P48169 Human
- Unigene: 248112 Human
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别名
- GABA(A) receptor subunit alpha 4 antibody
- GABA(A) receptor subunit alpha-4 antibody
- GABR A4 antibody
see all
图片
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Anti-GABRA4 antibody (ab117080) at 0.1 µg/ml + Human Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 62 kDa -
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab117080 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab117080 被引用在 3 文献中.
- Hughes BA et al. Chronic Ethanol Exposure and Withdrawal Impair Synaptic GABAA Receptor-Mediated Neurotransmission in Deep-Layer Prefrontal Cortex. Alcohol Clin Exp Res 43:822-832 (2019). PubMed: 30860602
- Bohnsack JP et al. Differential regulation of synaptic and extrasynaptic a4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology 105:124-132 (2016). PubMed: 26767953
- Van Skike CE et al. Chronic intermittent ethanol exposure produces persistent anxiety in adolescent and adult rats. Alcohol Clin Exp Res 39:262-71 (2015). PubMed: 25684048