Anti-GABA A Receptor alpha 1抗体[N95/35] (ab94585)
Key features and details
- Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-GABA A Receptor alpha 1抗体[N95/35]
参阅全部 GABA A Receptor alpha 1 一抗 -
描述
小鼠单克隆抗体[N95/35] to GABA A Receptor alpha 1 -
宿主
Mouse -
特异性
ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Cynomolgus monkey, Orangutan -
免疫原
Recombinant fragment within Mouse GABA A Receptor alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P62812 -
阳性对照
- WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.
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常规说明
The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N95/35 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use a concentration of 0.1 - 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
靶标
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功能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
疾病相关
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2554 Human
- Entrez Gene: 14394 Mouse
- Entrez Gene: 29705 Rat
- Omim: 137160 Human
- SwissProt: P08219 Cow
- SwissProt: P14867 Human
- SwissProt: P62812 Mouse
- SwissProt: P62813 Rat
see all -
别名
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
图片
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Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.
Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
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Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 52 kDaBlock: 1% BSA.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab94585 被引用在 7 文献中.
- Heiney K et al. Neuronal avalanche dynamics and functional connectivity elucidate information propagation in vitro. Front Neural Circuits 16:980631 (2022). PubMed: 36188125
- Zhou Y et al. Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice. Biosci Trends 15:330-340 (2021). PubMed: 34526443
- Bian Z et al. Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation. Front Pharmacol 12:752211 (2021). PubMed: 35002696
- Bu X et al. 1% Isoflurane and 1.2 µg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons. Front Aging Neurosci 12:591938 (2020). PubMed: 33304268
- Gong JL et al. The increased expression of GABA receptors within the arcuate nucleus is associated with high intraocular pressure. Mol Vis 24:574-586 (2018). PubMed: 30174387
- Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . PubMed: 25987849
- Tan S et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . PubMed: 21712993