Anti-G蛋白beta subunit like/GBL抗体(ab25974)
Key features and details
- Rabbit polyclonal to G protein beta subunit like/GBL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-G蛋白beta subunit like/GBL抗体
参阅全部 G protein beta subunit like/GBL 一抗 -
描述
兔多克隆抗体to G蛋白beta subunit like/GBL -
宿主
Rabbit -
特异性
ab25974 recognises GßL (G protein beta protein subunit-like). -
经测试应用
适用于: WBmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human G protein beta subunit like/GBL (C terminal).
(Peptide available asab39766) -
阳性对照
- WB: Human brain cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766).
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说明 |
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WB
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766). |
靶标
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功能
Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. -
组织特异性
Broadly expressed, with highest levels in skeletal muscle, heart and kidney. -
序列相似性
Belongs to the WD repeat LST8 family.
Contains 7 WD repeats. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 64223 Human
- Omim: 612190 Human
- SwissProt: Q9BVC4 Human
- Unigene: 29203 Human
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别名
- G protein beta subunit like antibody
- G protein beta subunit-like antibody
- Gable antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab25974 被引用在 1 文献中.
- Lin Z et al. Construction of competitive endogenous RNA network reveals regulatory role of long non-coding RNAs in type 2 diabetes mellitus. J Cell Mol Med 21:3204-3213 (2017). PubMed: 28643459