Application
Western blot
Sample
Human Cell lysate - other (SCLC - NCI-H146)
Loading amount
80 µg
Specification
SCLC - NCI-H146
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-0.1%Tween20 + 4%Milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL advance
Exposure
10 second(s)
Bands
Specific: 90 kDa
Additional data
Additional Notes
non-specific banding when over-exposed: 130, 60, 55, 40, 35, 25 and 23 kd
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 14 2011