Anti-Frizzled 5抗体(ab14475)
Key features and details
- Rabbit polyclonal to Frizzled 5
- Suitable for: ICC/IF, IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Frizzled 5抗体
参阅全部 Frizzled 5 一抗 -
描述
兔多克隆抗体to Frizzled 5 -
宿主
Rabbit -
特异性
Recognizes human Fz5. -
经测试应用
适用于: ICC/IF, IHC-FoFr, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide:
C-TGRTPPGPAATYHKQVSLSHV
conjugated to KLH, corresponding to N terminal amino acids 564-585 of Human Frizzled 5. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol, 0.609% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20530549
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20530549
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
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说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 20530549 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20530549 |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. |
靶标
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功能
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway. -
序列相似性
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
结构域
The PDZ-binding motif mediates interaction with GOPC.
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 7855 Human
- Entrez Gene: 14367 Mouse
- Entrez Gene: 317674 Rat
- Omim: 601723 Human
- SwissProt: Q13467 Human
- SwissProt: Q9EQD0 Mouse
- SwissProt: Q8CHL0 Rat
- Unigene: 17631 Human
see all -
别名
- C2orf31 antibody
- DKFZP434E2135 antibody
- Frizzled 5 seven transmembrane spanning receptor antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab14475 被引用在 6 文献中.
- Zhang L et al. Silencing of Long Noncoding RNA SOX21-AS1 Relieves Neuronal Oxidative Stress Injury in Mice with Alzheimer's Disease by Upregulating FZD3/5 via the Wnt Signaling Pathway. Mol Neurobiol 56:3522-3537 (2019). PubMed: 30143969
- Skaria T et al. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells. Cell Adh Migr 11:24-38 (2017). PubMed: 27159116
- Ma M et al. Wnt signaling in form deprivation myopia of the mice retina. PLoS One 9:e91086 (2014). WB ; Mouse . PubMed: 24755605
- Ye Z et al. Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension. Nat Commun 4:2803 (2013). PubMed: 24335906
- Sahores M et al. Frizzled-5, a receptor for the synaptic organizer Wnt7a, regulates activity-mediated synaptogenesis. Development 137:2215-25 (2010). ICC/IF, IHC-Fr ; Mouse, Rat . PubMed: 20530549
- Pereira C et al. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler Thromb Vasc Biol 28:504-10 (2008). PubMed: 18174455