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Question (75946) | Free Fatty Acid Assay Kit - Quantification (ab65341)

How would I account for endogenous long-chain Acyl-CoAs that are already in my tissue extract samples?

You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogenous Acyl-CoAs, you can subtract this background from the +ACS Reagent wells.