Free Fatty Acid Assay试剂盒- Quantification (ab65341)

概述

  • 产品名称
    Free Fatty Acid Assay试剂盒- Quantification
    参阅全部 Fatty acid 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    > 2 µM
  • 检测时间
    0h 40m
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Species independent
  • 产品概述

    Abcam's Free Fatty Acid Quantification Assay Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • 说明

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab65341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

实验方案

文献

This product has been referenced in:
  • Harm S  et al. Removal of stabilizers from human serum albumin by adsorbents and dialysis used in blood purification. PLoS One 13:e0191741 (2018). Human . Read more (PubMed: 29364955) »
  • Shimazu T  et al. Role of METTL20 in regulating ß-oxidation and heat production in mice under fasting or ketogenic conditions. Sci Rep 8:1179 (2018). Read more (PubMed: 29352221) »

See all 31 Publications for this product

客户评价及客户问答

I have had a response from the lab about you inquiry concerning the detection bandwidth for the ab6534 kit. Please check if your filter has a bandwidth option. If it does have a +/- 10 bandwidth option you can use this filter and still capture 100% of ...

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As you have detailed the kit measures FFA content in various biological samples (serum, plasma and other body fluids, food, growth media, etc) by enzymatically converting the fatty acids to their CoA derivatives with are oxidised ant their subsequent c...

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This kit can be used with tissue samples. The tissue preparation instructions are as follows: 10 mg tissue samples can be extracted by homogenization with 200 µl of chloroform-Triton X-100 (1% Triton X-100 in pure chloroform) in a microhomogenizer...

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We can confirm that this Kit will not be suitable to use with fatty acids less than 8 carbons or short chain fatty acids. This kit can detect Octanate or long chain fatty irrespective of species of the organism.

31-34 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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