Free Fatty Acid Assay试剂盒- Quantification (ab65341)

概述

  • 产品名称
    Free Fatty Acid Assay试剂盒- Quantification
    参阅全部 Fatty acid 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    > 2 µM
  • 检测时间
    0h 40m
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Species independent
  • 产品概述

    Abcam's Free Fatty Acid Quantification Assay Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • 说明

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab65341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

实验方案

文献

This product has been referenced in:
  • Okumura T  et al. Extra-pancreatic invasion induces lipolytic and fibrotic changes in the adipose microenvironment, with released fatty acids enhancing the invasiveness of pancreatic cancer cells. Oncotarget 8:18280-18295 (2017). Mouse . Read more (PubMed: 28407685) »
  • Domingo-Espín J  et al. Dual Actions of Apolipoprotein A-I on Glucose-Stimulated Insulin Secretion and Insulin-Independent Peripheral Tissue Glucose Uptake Lead to Increased Heart and Skeletal Muscle Glucose Disposal. Diabetes 65:1838-48 (2016). Read more (PubMed: 27207515) »

See all 25 Publications for this product

客户评价及客户问答

This kit will only detect the unbound free fatty acids in you sample and not any albumin bound free fatty acids,


The Free Fatty Acid Quantification Kit (ab65341) allows for the quantification of long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media. It is able to measure chain lengths of C8...

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Unfortunately, the components of the buffer in question are considered proprietary. However, the lab has asked that if you have a specific question to ask about the buffer, we will try to do our best to address the problem.

Yes, ab65341 has flexible uses so it can be used for both colorimetric and fluorometric, giving the user the choice and convenience of being able to perform and analyze both output types.

The “ACS” stands for Acyl-CoA synthesis, as in ACS reagent would be Acyl-CoA synthesis reagent.

I regret to inform you that I am not allowed to disclose the composition of this reagent due to proprietary restrictions.

Fa...

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The assay buffer used in ab65341 contains NP-40.



1.) Since the fluorometric assay is more sensitive than the colorimetric one, your standards might just plateau off if a higher concentration for the standard curve is used. I would say that 1 nmol/well of the standard as mentioned in the dat...

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Yes, this kit can be used for all C-8 (octanoate) and longer fatty acids assay. Since oleic acid fits into this category, you can use this assay kit. But please leer in mind that any other long chain fatty acids also present in the cells would be assay...

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As some of the components are quite sensitive like Fatty Acid enzyme mix, I am afraid that the kit will rather not work. However, they may be lucky: they could only perform an ELISA with the standard to check if the components are still working.

11-20 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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