Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis of E14.5 mouse embryos)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-based solution
Permeabilization
Yes - 0.2% gelatin, 0.25% Triton X-100 in PBS 1×
Specification
Testis of E14.5 mouse embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1% BSA
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Donkey Alexa Fluor® 488 conjugate anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sami Zaqout
Verified customer
提交于 Oct 03 2016