Application
Western blot
Sample
Mouse Tissue lysate - whole (fetal lung)
Loading amount
20 µg
Specification
fetal lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: IRDye800CW Anti-Mouse (LICOR)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/20000
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 50, 60 kDa
Additional data
Additional Notes
Fetal mouse lung lysate was used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 21 2013