Anti-Fluorescein抗体[6A4] (ab6213)
Key features and details
- Mouse monoclonal [6A4] to Fluorescein
- Suitable for: WB, ELISA, ICC
- Reacts with: Species independent
- Isotype: IgM
概述
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产品名称
Anti-Fluorescein抗体[6A4]
参阅全部 Fluorescein 一抗 -
描述
小鼠单克隆抗体[6A4] to Fluorescein -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, ICCmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Fluorescein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
6A4 -
骨髓瘤
unknown -
同种型
IgM -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/5000.
|
|
ELISA |
1/2000 - 1/20000.
|
|
ICC |
1/200 - 1/2000.
|
说明 |
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WB
1/500 - 1/5000. |
ELISA
1/2000 - 1/20000. |
ICC
1/200 - 1/2000. |
靶标
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相关性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
别名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab6213 被引用在 2 文献中.
- Maheswaranathan M et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . PubMed: 21857021
- Odagi I et al. Examination of normal intestine using confocal endomicroscopy. J Gastroenterol Hepatol 22:658-62 (2007). PubMed: 17444852