Anti-Flagellin抗体(ab93713)
Key features and details
- Rabbit polyclonal to Flagellin
- Suitable for: WB, ICC/IF
- Reacts with: Escherichia coli, Salmonella anatum, Salmonella selandia, Salmonella
- Isotype: IgG
概述
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产品名称
Anti-Flagellin抗体
参阅全部 Flagellin 一抗 -
描述
兔多克隆抗体to Flagellin -
宿主
Rabbit -
特异性
This antibody is specific for bacterial Flagellin (FliC). -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Escherichia coli, Salmonella anatum, Salmonella selandia, Salmonella -
免疫原
Purified recombinant Flagellin made in BL21 bacteria.
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阳性对照
- Lysates transfected with purified flagellin, E. Coli total lysate or 293T transfected with recombinant protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/10000 - 1/20000. Detects a band of approximately 60 kDa.
|
ICC/IF |
1/100.
|
说明 |
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WB
1/10000 - 1/20000. Detects a band of approximately 60 kDa. |
ICC/IF
1/100. |
靶标
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相关性
Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs). -
细胞定位
Secreted. Bacterial flagellum. -
数据库链接
- Entrez Gene: 949101 Escherichia coli
- Entrez Gene: 961921 Escherichia coli
- SwissProt: P04949 Escherichia coli
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别名
- 41 kDa antigen antibody
- b1923 antibody
- flaF antibody
see all
图片
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All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilution
All lanes : 293T transfected with recombinant protein
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI. -
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab93713 被引用在 27 文献中.
- Gao F et al. Calreticulin (CALR)-induced activation of NF-ĸB signaling pathway boosts lung cancer cell proliferation. Bioengineered 13:6856-6865 (2022). PubMed: 35264066
- Liu T et al. The Rcs System Contributes to the Motility Defects of the Twin-Arginine Translocation System Mutant of Extraintestinal Pathogenic Escherichia coli. J Bacteriol 204:e0061221 (2022). PubMed: 35311558
- Cohen H et al. Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium. PLoS Pathog 18:e1010425 (2022). PubMed: 35381053
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Li F et al. Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution. NPJ Biofilms Microbiomes 8:39 (2022). PubMed: 35546554