Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (gel 8%)
Sample
Human Cell lysate - whole cell (ovarian cancer cell line: SKOV3)
Specification
ovarian cancer cell line: SKOV3
Blocking step
Blocking one (nacalai tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: can get signal (TOYOBO) solution1
Dilution
1/50000
Secondary antibody
Name
Non-Abcam antibody was used: sc-2004 goat anti rabbit santacruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 263 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
PubMed ID
23856093: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 18 2013