重组Anti-Fibrinogen gamma chain抗体[EPR3084] (ab92481)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3084] to Fibrinogen gamma chain
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Fibrinogen gamma chain抗体[EPR3084]
参阅全部 Fibrinogen gamma chain 一抗 -
描述
兔单克隆抗体[EPR3084] to Fibrinogen gamma chain -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human plasma lysate IHC-P: Human liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3084 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | ||
IHC-P |
说明 |
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WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
疾病相关
Defects in FGG are a cause of thrombophilia.
Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻译后修饰
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2266 Human
- Omim: 134850 Human
- SwissProt: P02679 Human
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别名
- FGG antibody
- FIBG_HUMAN antibody
- Fibrinogen gamma chain antibody
see all
图片
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Anti-Fibrinogen gamma chain antibody [EPR3084] (ab92481) at 1/500 dilution + Human
plasma lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 52 kDa -
ab92481, at a 1/100 dilution, staining Fibrinogen gamma chain in paraffin embedded Human liver tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab92481 被引用在 1 文献中.
- Calvo E et al. Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts. J Pers Med 10:N/A (2020). PubMed: 33096722