Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Rat optic nerve head)
Antigen retrieval step
None
Permeabilization
Yes - 0.05% Triton in PBS
Specification
Rat optic nerve head
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% serum in phosphate buffered saline
Dilution
1/400
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Jackson Cy™3 AffiniPure Donkey Anti-Sheep IgG (H+L
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
Image: fibrinogen accumulation after ischemic injury fibrinogen is shown in yellow, red marks are laminin,
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提交于 Jan 26 2017