Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: DAKO Antibody diluent
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector labs
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for taking part in our AbTrial your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in rat which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 28 2016