Application
Western blot
Sample
Mouse Tissue lysate - other (Brain)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Brain
Blocking step
Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 45% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer (PBS)+Tween20
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Licor IRDye 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
Odyssey
Negative control
Cytoplasmic Fraction
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 75 kDa
Positive control
Nuclear Fraction
Additional data
Additional Notes
This was my first test the antibody also works at lower concentrations (1:1000).
Lanes 1&2: Total Lysate
Lanes 3&4: Cytoplasmic Fraction
Lanes 5&6: Nuclear Fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 18 2015