Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Eye, muscle)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel, NuPAGE)
Loading amount
23 µg
Specification
Eye, muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: sc-2004, Santa Cruz goat-anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
None
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 22, 24, 27 kDa Non-specific: 37, very faint kDa
Positive control
Jurkat cell lysate showed positive control band at 22 kDa
Additional data
Additional Notes
Membrane: PVDF
Lysis buffer: RIPA (BP-115X, Boston Bioproducts)
Protease Inhibitor: Roche cOmplete EDTA-free mini tablets
1. PageRuler Plus Prestained Ladder, 10 to 250 kDa, Thermo Fisher
2. Adult fish #1, eye
3. Adult fish #1, muscle
4. Adult fish #2, eye
5. Adult fish #2, muscle
6. Jurkat cell lysate
Molecular weight markers from top to bottom: 250, 130, 100, 70, 55, 35, 25, 15, 10 (10 is very faint) kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Cheri Ackerman
Verified customer
提交于 Aug 11 2016