Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (TGX any size gel)
Sample
Fish Cell lysate - whole cell (Liver)
Specification
Liver
Blocking step
(agent) for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS/1% casein
Dilution
1/1500
Detection
Detection method
Pierce ECL 2
Bands
Specific: ~21 kDa
Exposure
3 minute(s) and 0 second(s)
Positive control
5,25,50,100,200 nanograms high purity human liver Ferritin
Additional data
Additional Notes
Lane 1 - Antarctic Notothenioid Fish Species #1
Lane 2 - Antarctic Notothenioid Fish Species #2
Lane 3 - Antarctic Notothenioid Fish Species #3
Lane 4 - Antarctic Notothenioid Fish Species #4
Lane 5 - 5.0 ng high purity human liver Ferritin
Lane 6 - 25.0 ng high purity human liver Ferritin
Lane 7 - 50.0 ng high purity human liver Ferritin
Lane 8 - 100 ng high purity human liver Ferritin
Lane 9 - 200 ng high purity human liver Ferritin
Liver samples homogenized at 10:1 (v:w) in RIPA buffer
PVDF membranes
BioRad's Image Lab used to visualize
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Don Kuhn
Verified customer
提交于 Oct 29 2013