Anti-Ferritin Light Chain抗体(ab69090)
Key features and details
- Rabbit polyclonal to Ferritin Light Chain
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Ferritin Light Chain抗体
参阅全部 Ferritin Light Chain 一抗 -
描述
兔多克隆抗体to Ferritin Light Chain -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: IHC-FoFr, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog, Orangutan -
免疫原
Synthetic peptide corresponding to Human Ferritin Light Chain aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab69089) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69090于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-FoFr | (1) |
Use a concentration of 1 µg/ml.
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WB | (21) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa).
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说明 |
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IHC-FoFr
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa). |
靶标
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功能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
疾病相关
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
序列相似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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数据库链接
- Entrez Gene: 477042 Dog
- Entrez Gene: 2512 Human
- Entrez Gene: 14325 Mouse
- Entrez Gene: 100173858 Orangutan
- Entrez Gene: 29292 Rat
- Omim: 134790 Human
- SwissProt: Q53VB8 Dog
- SwissProt: P02792 Human
see all -
别名
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
图片
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All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 :Mouse spleen tissue lysate - total protein (ab29293)
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lane 5 : Liver (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (78)
ab69090 被引用在 78 文献中.
- Cheng Z et al. Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis. Cancer Sci 113:65-78 (2022). PubMed: 34699654
- Tisdall MD et al. Ex vivo MRI and histopathology detect novel iron-rich cortical inflammation in frontotemporal lobar degeneration with tau versus TDP-43 pathology. Neuroimage Clin 33:102913 (2022). PubMed: 34952351
- Yue L et al. PCBP2 knockdown promotes ferroptosis in malignant mesothelioma. Pathol Int 72:242-251 (2022). PubMed: 35089637
- Raha AA et al. Interplay of Ferritin Accumulation and Ferroportin Loss in Ageing Brain: Implication for Protein Aggregation in Down Syndrome Dementia, Alzheimer's, and Parkinson's Diseases. Int J Mol Sci 23:N/A (2022). PubMed: 35162984
- Michaelis V et al. Differences and Interactions in Placental Manganese and Iron Transfer across an In Vitro Model of Human Villous Trophoblasts. Int J Mol Sci 23:N/A (2022). PubMed: 35328723