Application
Flow Cytometry
Sample
Human Cell (basophil granulocytes)
Permeabilization
No
Gating Strategy
leuko gate + CD123 positive cells
Specification
basophil granulocytes
Preparation
Cell harvesting/tissue preparation method: separated by Percoll density separation, followed by magnetic isolation for basophils
Sample buffer: PBS with human IgG (Fc-block)
Sample buffer: PBS with human IgG (Fc-block)
Fixation
no fixation
Other product details
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: PBS with human IgG (Fc-block)
Concentration
10 µl/cells
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: goat anti-mouse FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
lanes:
basophile granulocytes:
1 = Isotype
2 = unstimulated
3 = stimulated with a-IgE
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 25 2012