Anti-Factor VIII抗体(ab61370)
Key features and details
- Sheep polyclonal to Factor VIII
- Suitable for: ELISA, Inhibition Assay
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Factor VIII抗体
参阅全部 Factor VIII 一抗 -
描述
羊多克隆抗体to Factor VIII -
宿主
Sheep -
经测试应用
适用于: ELISA, Inhibition Assaymore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length Human Factor VIII
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 50% Water, 50% Glycerol (glycerin, glycerine)
50% (v/v)Glycerol/Water -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
Salt fractionation followed by ion exchange chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Inhibition Assay |
Use at an assay dependent concentration.
Binds FVIII/vWF complex in plasma. |
说明 |
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ELISA
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. Binds FVIII/vWF complex in plasma. |
靶标
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功能
Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. -
疾病相关
Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. -
序列相似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
结构域
Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity. -
翻译后修饰
Sulfation on Tyr-1699 is essential for binding vWF. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 2157 Human
- Omim: 300841 Human
- SwissProt: P00451 Human
- Unigene: 654450 Human
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别名
- AHF antibody
- Antihemophilic factor antibody
- Coagulation factor VIII antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab61370 被引用在 2 文献中.
- Link KG et al. A mathematical model of coagulation under flow identifies factor V as a modifier of thrombin generation in hemophilia A. J Thromb Haemost 18:306-317 (2020). PubMed: 31562694
- Wang Q et al. Comparison of human coagulation factor VIII expression directed by cytomegalovirus and mammary gland-specific promoters in HC11 cells and transgenic mice. Blood Coagul Fibrinolysis 26:755-61 (2015). ELISA . PubMed: 26192111