Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Intestine)
Loading amount
20 µg
Specification
Brain, Intestine
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 10 kDa Non-specific: 20, 30, 35, 40 kDa
Additional data
Abcam response
Thank you for your valuable feedback. Mouse is not a tested species for ab76597 but your data suggest that ab76597 recognizes mouse FXYD1 (along with multiple background bands) following your experimental conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 04 2010