Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton X-100
Specification
Brain
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS+0.4% Triton X-100+10% BSA+3% serum
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Additional Notes
Staining pattern using ab16046 (pseudo-colored blue) is not consistent with known expression pattern of Foxp2 in cortex, shown as nuclear staining in cortical layer 6 using Santa Cruz Biotechnology anti-FOXP2 N-16. The non-nuclear staining with N-16 in Foxp2-cKO is non-specific blood vessel staining commonly seen with this antibody in our hands. Furthermore, no nuclear Foxp2 staining is seen in WT hippocampus using N-16, consistent with in situ hybridization data (Ferland et al 2003), but nuclear staining is abundant in WT hippocampus using ab16046. Therefore we believe ab16046 is not specific for Foxp2 when used for IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Marissa Co
Verified customer
提交于 Dec 28 2017