Application
Western blot
Sample
Rat Cell lysate - whole cell (rat vscular smooth muscle cells)
Loading amount
10 µg
Specification
rat vscular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5%BSA+0.1% Tween 20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL plus
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 105 kDa
Additional data
Additional Notes
lane 1, rat VSMC, lane 2, rat cells with activated AKT.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 04 2011