Application
Western blot
Sample
Human Cell lysate - whole cell (293 FT cell line)
Loading amount
50 µg
Specification
293 FT cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 50~75, 100~150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 25 2010