Application
Western blot
Sample
Sheep Cell lysate - whole cell (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8% bisacrylamide gels)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG (H+L) HRP secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 6 second(s)
Bands
Specific: 70 kDa Non-specific: 30, 40, 60 kDa
Positive control
Protein lysate from adult rat vastus lateralis was used. Also had a non-specific band at 30 and 60 kDa.
Additional data
Additional Notes
Lane 1: Adult sheep vastus lateralis tissue
Lane 2: Fetal sheep vastus lateralis tissue
Lane 3: Adult rat vastus lateralis tissue
A nitrocellulose membrane was used.
RIPA buffer was used to isolate proteins. Samples were boiled in 1x Laemmli buffer at 95 degrees C for five minutes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 13 2018